This hands-on activity is an assessment of the students understanding of peptide and disulfide bonds formed during protein synthesis, and the structure of an amino acid (R-group plus the common structure that all amino acids share). Students will demonstrate the process of dehydration synthesis by combining amino acids. They will synthesize one molecule of the protein ADH (antidiuretic hormone) by reading the mRNA sequence, cutting out the -OH on the carboxyl group on amino acid one, and the -H on the amino group of amino acid two. These two pieces (-OH and -H) will combine to form a water molecule. The students will connect the two amino acids by taping a peptide bond label between the carbon of the carboxyl and the nitrogen of the amino group on amino acid. This process is continued until the stop codon on the mRNA is read. If the synthesis is done correctly, the student will produce eight peptide bonds creating one chain, and eight molecules of water. If this activity is used for biology, the tRNA, and rRNA molecules can be added to the process.